Cyanogenic Potential and Partial Purification of (3-Glucosidase from Time 419 Cultivar:- Nwoji, Jospeh O.
ABSTRACT
Cyanogenic potential and partial
purification of p-glucosidase was carried out on cassava cultivar TME 419 and
other processed cassava food using acceptable chemical methods. Extraction
using acetate buffer and precipitating with ammonium sulfate S04) gave a
partially purified enzyme with enzyme activity of4.5 unit/ml and specific
activity of 1.248 unit/mg protein. Using the protocol B method for total
cyanide determination, the following was found to be the total cyanide
distribution in both wet and diy peel, pulp and leave (120.78ppm, 26.14ppm and
75.24ppm) and (25.34ppm, 53.46ppm and 24.16ppm) respectively with carotenoid
value of 7mg/g in the pulp ofthe cassava cultivar TME 419. The protein content
ofthe partially purified enzyme was 3.6038mg/ml
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NWOJI, O (2023). Cyanogenic Potential and Partial Purification of (3-Glucosidase from Time 419 Cultivar:- Nwoji, Jospeh O.. Mouau.afribary.org: Retrieved Nov 17, 2024, from https://repository.mouau.edu.ng/work/view/cyanogenic-potential-and-partial-purification-of-3-glucosidase-from-time-419-cultivar-nwoji-jospeh-o-7-2
OBIOMA, NWOJI. "Cyanogenic Potential and Partial Purification of (3-Glucosidase from Time 419 Cultivar:- Nwoji, Jospeh O." Mouau.afribary.org. Mouau.afribary.org, 27 Oct. 2023, https://repository.mouau.edu.ng/work/view/cyanogenic-potential-and-partial-purification-of-3-glucosidase-from-time-419-cultivar-nwoji-jospeh-o-7-2. Accessed 17 Nov. 2024.
OBIOMA, NWOJI. "Cyanogenic Potential and Partial Purification of (3-Glucosidase from Time 419 Cultivar:- Nwoji, Jospeh O.". Mouau.afribary.org, Mouau.afribary.org, 27 Oct. 2023. Web. 17 Nov. 2024. < https://repository.mouau.edu.ng/work/view/cyanogenic-potential-and-partial-purification-of-3-glucosidase-from-time-419-cultivar-nwoji-jospeh-o-7-2 >.
OBIOMA, NWOJI. "Cyanogenic Potential and Partial Purification of (3-Glucosidase from Time 419 Cultivar:- Nwoji, Jospeh O." Mouau.afribary.org (2023). Accessed 17 Nov. 2024. https://repository.mouau.edu.ng/work/view/cyanogenic-potential-and-partial-purification-of-3-glucosidase-from-time-419-cultivar-nwoji-jospeh-o-7-2