Chemical investgation of hariingana madagascariensis lam. Ex. Poir (blood tree), sarcocephalus esculentus afzel(african peach) and baphia nittda(camwood) as anti-TB agents:- Okoro, Ijeoma
ABSTRACT
In this investigation, the plants Haraungana
madagascariensis, Sarcocephalus esculentus and Baphia nitida were selected on
the basis of their anti TB uses as claimed by the traditional healers/
practioners. The plants were extracted by percolation using ethanol. The
residues ETi, ET2, and ET3 from Harungana madagascariensis, sarcocephalus
esculentus and Baphia nitida respectively were partitioned and concentrated to
obtain fractions (Chi, Chz, and Chs), petroleum ether fractions(PEi, PE2, and
PE3), aqueous methanol fractions(MEi,ME2, and ME3) and water fractions of (WAi,
WA2, and WA3), obtained were subjected to antimicrobial screening assay (AMA)
using agar diffusion method. The chloroform fractions Chi, Chz, and Cha 1 .
being relatively active in all tests were made candidates for chromatography.
Column chromatography of the fraction Chi, from H. madagascariensis afforded
three relatively pure compounds IJFi, IJGi, and IJHi, while fraction Chz from
S. esculentus gave two pure compounds IJEi, and IJF2 and fraction from B.
nitida afforded one compound IJI2. The purity of these compounds were confirmed
using different solvents with varying polarities. A combination of mass spectra
1HNMR, 13NMR, DEPT 45, 90 and 135, H/C, and COSY iH/1!! COSY helped in
proposing the structures. The results obtained in the (AMA) proved to be a good
pointer to the presence of active secondary metabolites in IJFi, IJGi, IJHi,
IJE2, IJF2, IJI2 and other fractions in varying proportions.
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IJEOMA, I (2024). Chemical investgation of hariingana madagascariensis lam. Ex. Poir (blood tree), sarcocephalus esculentus afzel(african peach) and baphia nittda(camwood) as anti-TB agents:- Okoro, Ijeoma . Mouau.afribary.org: Retrieved Jul 27, 2024, from https://repository.mouau.edu.ng/work/view/chemical-investgation-of-hariingana-madagascariensis-lam-ex-poir-blood-tree-sarcocephalus-esculentus-afzelafrican-peach-and-baphia-nittdacamwood-as-anti-tb-agents-okoro-ijeoma-7-2
IJEOMA, IJEOMA. "Chemical investgation of hariingana madagascariensis lam. Ex. Poir (blood tree), sarcocephalus esculentus afzel(african peach) and baphia nittda(camwood) as anti-TB agents:- Okoro, Ijeoma " Mouau.afribary.org. Mouau.afribary.org, 13 May. 2024, https://repository.mouau.edu.ng/work/view/chemical-investgation-of-hariingana-madagascariensis-lam-ex-poir-blood-tree-sarcocephalus-esculentus-afzelafrican-peach-and-baphia-nittdacamwood-as-anti-tb-agents-okoro-ijeoma-7-2. Accessed 27 Jul. 2024.
IJEOMA, IJEOMA. "Chemical investgation of hariingana madagascariensis lam. Ex. Poir (blood tree), sarcocephalus esculentus afzel(african peach) and baphia nittda(camwood) as anti-TB agents:- Okoro, Ijeoma ". Mouau.afribary.org, Mouau.afribary.org, 13 May. 2024. Web. 27 Jul. 2024. < https://repository.mouau.edu.ng/work/view/chemical-investgation-of-hariingana-madagascariensis-lam-ex-poir-blood-tree-sarcocephalus-esculentus-afzelafrican-peach-and-baphia-nittdacamwood-as-anti-tb-agents-okoro-ijeoma-7-2 >.
IJEOMA, IJEOMA. "Chemical investgation of hariingana madagascariensis lam. Ex. Poir (blood tree), sarcocephalus esculentus afzel(african peach) and baphia nittda(camwood) as anti-TB agents:- Okoro, Ijeoma " Mouau.afribary.org (2024). Accessed 27 Jul. 2024. https://repository.mouau.edu.ng/work/view/chemical-investgation-of-hariingana-madagascariensis-lam-ex-poir-blood-tree-sarcocephalus-esculentus-afzelafrican-peach-and-baphia-nittdacamwood-as-anti-tb-agents-okoro-ijeoma-7-2