ABSTRACT
Various microorganisms
are able to invade the urinary tract and can be involved in the
pathogenesis of urinary
tract infection. The isolation and identification of bacteria species
from urine samples of
Michael Okpara University of Agriculture students (MOUAU)
students and
determination of its antibiotics sensitivity were carried out. This revealed
that
out of forty-five (45)
urine samples examined from the male and female students of
MOUAU, thirty four (34)
showed positive growths at 73.3% frequency occurrence, while
eleven (11) samples
showed negative cultures at 24.4% frequency occurrence respectively. It
was also revealed that
a total of five (5) bacteria species were isolated and identified by their
morphological
characteristics, pigmentation on media, microscopy, and biochemical
methods. The bacterial
species isolated from the urine samples include Escherichia coli,
Klebsiella spp.
Staphylococcus aureus, Proteus spp and Coagulase Negative Staphylococcus
aureus respectively.
The distribution and percentage occurrence of bacterial isolates from the
urine specimens were
different. In the urine specimens from female individuals, the
predominant bacterial
isolates were Staphylococcus aureus 19(35.8%), followed by
Escherichia coli
17(32.1%) and Klebsiella spp 11(20.7%), while in male individuals the
predominant bacterial
isolates were Staphylococcus aureus 11(34.3%), followed by
Escherichia coli
9(28.1%) and Klebsiella spp 6(18.8%). It was also revealed in this study that
the antibiotic
susceptibility and resistant profile for bacteria isolates varied. Escherichia
coli
and Staphylococcus
aureus showed high level of sensitive to the antibiotics Rifampicin
(20mcg), Ciprofloxacin
(lOmcg), Ampiclox (20mcg), and Gentamycin (lOmcg), while the
resistant profile of
the isolates revealed that Escherichia coli and Staphylococcus aureus had
highest number of
isolates resistant to the antibiotic drug Rifampicin (20mcg) and
Chloramphenicol
(30mcg). This implies that these antibiotics cannot be used as empirical
therapy for urinary
tract infection particularly in the study area. However, Rifampicin
(20mcg), Ciprofloxacin
(lOmcg), Ampiclox (20mcg), and Gentamycin (lOmcg) antibiotic
were the most effective
drug against urinary tract infections, thus could be of alternative of
choice to use and to
control bacterial infection as an effective antibacterial agent.
AZUBUIKE, S (2025). Characterization And Antibiotic Susceptibility Profile Of Bacterial Isolates In Urine Samples From Michael Okpara University Of Agriculture Students:- Azubuike, Chioma S. Mouau.afribary.org: Retrieved Apr 03, 2025, from https://repository.mouau.edu.ng/work/view/characterization-and-antibiotic-susceptibility-profile-of-bacterial-isolates-in-urine-samples-from-michael-okpara-university-of-agriculture-students-azubuike-chioma-s-7-2
SUSAN, AZUBUIKE. "Characterization And Antibiotic Susceptibility Profile Of Bacterial Isolates In Urine Samples From Michael Okpara University Of Agriculture Students:- Azubuike, Chioma S" Mouau.afribary.org. Mouau.afribary.org, 24 Feb. 2025, https://repository.mouau.edu.ng/work/view/characterization-and-antibiotic-susceptibility-profile-of-bacterial-isolates-in-urine-samples-from-michael-okpara-university-of-agriculture-students-azubuike-chioma-s-7-2. Accessed 03 Apr. 2025.
SUSAN, AZUBUIKE. "Characterization And Antibiotic Susceptibility Profile Of Bacterial Isolates In Urine Samples From Michael Okpara University Of Agriculture Students:- Azubuike, Chioma S". Mouau.afribary.org, Mouau.afribary.org, 24 Feb. 2025. Web. 03 Apr. 2025. < https://repository.mouau.edu.ng/work/view/characterization-and-antibiotic-susceptibility-profile-of-bacterial-isolates-in-urine-samples-from-michael-okpara-university-of-agriculture-students-azubuike-chioma-s-7-2 >.
SUSAN, AZUBUIKE. "Characterization And Antibiotic Susceptibility Profile Of Bacterial Isolates In Urine Samples From Michael Okpara University Of Agriculture Students:- Azubuike, Chioma S" Mouau.afribary.org (2025). Accessed 03 Apr. 2025. https://repository.mouau.edu.ng/work/view/characterization-and-antibiotic-susceptibility-profile-of-bacterial-isolates-in-urine-samples-from-michael-okpara-university-of-agriculture-students-azubuike-chioma-s-7-2