Antimicrobial Activity Of Helianthus Annuus Leaves On Some Bacterial Pathogens (E.Coli, Salmonella Spp, Shigella Spp, And Staphylococcus Aureus)
ABSTRACT
The antibacterial activity of
Helianthus annuus leaves on some bacterial pathogens (E. coil, Salmonella spp,
Shigella spp, and Staphylococcus aureus) was evaluated. The extracts were
prepared using chloroform, N-hexane and methanol. The antibacterial
susceptibility testing of the extracts were carried out using the Agar well
diffusion method. The phytochemical analysis was done using standard method
while the antioxidant activity was determined using the
2,2-diphenyl-1-picrylhydrazyl (DPPH) photometric assay. The antibacterial
testing showed that the test organism were all susceptible to the extract. S.
aureus showed the highest susceptibility to the extracts while Shigella spp
showed least susceptibility. The Minimum inhibitory concentration of the
methanolic fraction of the extract was determined to be 125tgIml on all the
test organisms. The phytochemical analysis show the presence of Saponins,
Tannins, Glycoside, Alkaloids and Flavonoids. The extracts produced a
concentration dependent increase in antioxidant activity. The chloroform showed
greater antioxidant activity while the methanolic extract was least compared to
the standard solution or Ascorbic acid. This therefore gives a scientific
justification for the use of Helianthus annus leave on some bacterial pathogens
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EZE, A (2021). Antimicrobial Activity Of Helianthus Annuus Leaves On Some Bacterial Pathogens (E.Coli, Salmonella Spp, Shigella Spp, And Staphylococcus Aureus). Mouau.afribary.org: Retrieved Nov 16, 2024, from https://repository.mouau.edu.ng/work/view/antimicrobial-activity-of-helianthus-annuus-leaves-on-some-bacterial-pathogens-ecoli-salmonella-spp-shigella-spp-and-staphylococcus-aureus-7-2
ABEL, EZE. "Antimicrobial Activity Of Helianthus Annuus Leaves On Some Bacterial Pathogens (E.Coli, Salmonella Spp, Shigella Spp, And Staphylococcus Aureus)" Mouau.afribary.org. Mouau.afribary.org, 04 Aug. 2021, https://repository.mouau.edu.ng/work/view/antimicrobial-activity-of-helianthus-annuus-leaves-on-some-bacterial-pathogens-ecoli-salmonella-spp-shigella-spp-and-staphylococcus-aureus-7-2. Accessed 16 Nov. 2024.
ABEL, EZE. "Antimicrobial Activity Of Helianthus Annuus Leaves On Some Bacterial Pathogens (E.Coli, Salmonella Spp, Shigella Spp, And Staphylococcus Aureus)". Mouau.afribary.org, Mouau.afribary.org, 04 Aug. 2021. Web. 16 Nov. 2024. < https://repository.mouau.edu.ng/work/view/antimicrobial-activity-of-helianthus-annuus-leaves-on-some-bacterial-pathogens-ecoli-salmonella-spp-shigella-spp-and-staphylococcus-aureus-7-2 >.
ABEL, EZE. "Antimicrobial Activity Of Helianthus Annuus Leaves On Some Bacterial Pathogens (E.Coli, Salmonella Spp, Shigella Spp, And Staphylococcus Aureus)" Mouau.afribary.org (2021). Accessed 16 Nov. 2024. https://repository.mouau.edu.ng/work/view/antimicrobial-activity-of-helianthus-annuus-leaves-on-some-bacterial-pathogens-ecoli-salmonella-spp-shigella-spp-and-staphylococcus-aureus-7-2