ABSTRACT
The
antimicrobial activity of aqueous and ethanolic extract of leaf and seed of
piper guineense was determined on three bacteria {Staphylococcus aureus,
Escherichia coli and Salmonella spp) and three fungi (Aspergillus niger,
Aspergillus flavus, and Pencillium spp) using agar well diffusion method and
minimum inhibitory concentration (MIC). The ethanolic extract was found to show
more activity than the aqueous extract on the isolates. The diameter zone of
inhibition for the ethanolic extract (leaf and seed) range between 8 and 21mm
while that of aqueous extract ranged between 8 and 17mm. The MIC ofthe
ethanolic extract ranged from 3.125 to lOOmg/ml, while for aqueous extract, the
MIC was from 3.125 to 25mg/ml. Escherichia coli was found to show the greatest
sensitivity of all the isolates while Penicillium spp showed the least sensitivity
of all the isolates. This observed difference showed that Piper guineense
extracts contains antibacterial and antifungal compounds that may be useful for
the discovery of new antibiotics. The phytochemical analysis carried out on the
Piper guineense leaf and seed revealed the presence of alkaloids, tannis,
saponins, glycosides and flavonoids. The presence of these phytochemicals
supports the use ofthis plants as an antimicrobial agent.
ESTHER, E (2023). Antimicrobial Activities And Phytochemical Screening Of Piper Guineense (Uziza) Seed And Leaf Against Food Spoilage Organisms:- Ogbuagu, Esther. Mouau.afribary.org: Retrieved Nov 24, 2024, from https://repository.mouau.edu.ng/work/view/antimicrobial-activities-and-phytochemical-screening-of-piper-guineense-uziza-seed-and-leaf-against-food-spoilage-organisms-ogbuagu-esther-7-2
ESTHER, ESTHER. "Antimicrobial Activities And Phytochemical Screening Of Piper Guineense (Uziza) Seed And Leaf Against Food Spoilage Organisms:- Ogbuagu, Esther" Mouau.afribary.org. Mouau.afribary.org, 19 Oct. 2023, https://repository.mouau.edu.ng/work/view/antimicrobial-activities-and-phytochemical-screening-of-piper-guineense-uziza-seed-and-leaf-against-food-spoilage-organisms-ogbuagu-esther-7-2. Accessed 24 Nov. 2024.
ESTHER, ESTHER. "Antimicrobial Activities And Phytochemical Screening Of Piper Guineense (Uziza) Seed And Leaf Against Food Spoilage Organisms:- Ogbuagu, Esther". Mouau.afribary.org, Mouau.afribary.org, 19 Oct. 2023. Web. 24 Nov. 2024. < https://repository.mouau.edu.ng/work/view/antimicrobial-activities-and-phytochemical-screening-of-piper-guineense-uziza-seed-and-leaf-against-food-spoilage-organisms-ogbuagu-esther-7-2 >.
ESTHER, ESTHER. "Antimicrobial Activities And Phytochemical Screening Of Piper Guineense (Uziza) Seed And Leaf Against Food Spoilage Organisms:- Ogbuagu, Esther" Mouau.afribary.org (2023). Accessed 24 Nov. 2024. https://repository.mouau.edu.ng/work/view/antimicrobial-activities-and-phytochemical-screening-of-piper-guineense-uziza-seed-and-leaf-against-food-spoilage-organisms-ogbuagu-esther-7-2