ABSTRACT

Infections caused by s.aureus is of public health concern due to the easy acquisition of resistance genes. In this research study, the antibiotic sensitivity pattern of clinical isolates of S. aureus in vitro was studied against 3 groups of antibiotics namely quinolones, cephalosporins and aminoglycosides, Susceptibility and resistance were compared and analysed using the disc diffusion method with forty clinical isolates of S.aureus from different specimens ranging from urine to wound and others from hospitals in umuahia. Quinolones were observed to have the greatest antibiotic activity on S. aureus in vitro. S.aureus was found to be most sensitive to pefloxacin (85%) followed by ciprofloxacin (80%). While cephalosporins showed 65% sensitivity, cefuroxin was observed to show 60%. The Aminoglycosides had reduced sensitivity than the other antibiotics used. 

TABLE OF CONTENT

Title page i

Certification ii

Declaration iii

Dedication iv

Acknowledgement v

Table of Content vi

List of Table ix

Abstract x

CHAPTER ONE: INTRODUCTION

1.1 BACKGROUND 1

1.2 IMPORTANCE OF RESEARCH 3

1.3. THE AIM AND OBJECTIVE OF RESEARCH 4

1.4 OBJECTIVE 4

CHAPTER TWO: LITERATURE REVIEW

2.0. THE STAPHYLOCOCCI 6

2.1. IMPORTANT PROTERTIES OF STAPHYLOCOCCI 6

 2.2. STAPHYLOCOCCUS AUREUS 7

2.3. ROLE IN DISEASE 8

2.4. SKIN INFECTIONS 8

2.5. BACTEREMIA. 9

 2.6. ANIMAL INFECTIONS 9

2.7. VIRULENCE FACTORS 10

2.8. STAPHYLOCOCCAL PIGMENTS 12

2.9. CLASSICAL DIAGNOSIS 13

2.10. TREATMENT AND ANTIBIOTIC RESISTANCE 13

2.11. MECHANISM OF RESISTANCE 15

2.12. CARRIAGE OF STAPHYLOCOCCUS AUREUS 18

2.13. INFECTION CONTROL 18

CHAPTER THREE: MATERIALS AND METHOD

3.1. SAMPLES 20

3.2. DISC DIFFUSION METHOD 20

CHAPTER FOUR: RESULT PRESENTATION

4.1 Results 21

CHAPTER FIVE

5.1 DISCUSSION 27

5.2 CONCLUSION 30

5.3 RECOMMENDATION 30

Reference

Appendix