ABSTRACT

The aim of this study is to assess the antibiogram of microorganisms involved in the degradation of Abattoir in Umuahia, Abia State. A total of eight (8) samples which include (Soil, Waste water, Knife, Table swab, Wall swab, Horns, Feaces and Hand swab) were collected into a sterile containers and with the aid of sterile swab stick and was immediately transported to Michael Okpara University of Agriculture Microbiology Laboratory for bacteriological analysis. Isolation of microorganisms from the abattoir effluent samples were aseptically carried out using standard microbiological pour plate and streaking method on MacConkey agar (MA) and Nutrient agar (NA), Salmonella-Shigella Agar and Mannitol Salt agar respectively. A total of nine (9) microbial genera were identified after standard biochemical and cultural test was done which six (6) were bacterial species (Bacillus sp, Escherichia coli, Serratia sp, Pseudornonas cieruginosci, Proteus sp, Micrococcu sp), and three (3) were fungal specie (Aspergillus flcvus, Rhizoptis stolonifer and Rhodotorula sp). The total bacteria count from the soil and feaces weren7.7 x lO cfulg and 8.8 x i05 cfulg respectively while the total flingal counts from the soil and faeces were 2.9 x cfulg and 4.0 x l0 cfti/g respectively. The percentage occurrence of the bacterial isolates showed that E.coli 28% had the highest percentage occurrence while Pseucloinoncis aeruginosa had the least percentage occurrence of 4%. The percentage occurrence of fungal isolates showed that A. flavus had the highest occurrence of 46% while Rhodotorula sp showed the least occurrence of 23%. The Antibiogram patterns of the bacteria isolates revealed that Micrococcu sp and Pseudomonas aeruginosa were 100% sensitive to ciprofioxacin and Norfioxacin while Bacillus species and Micrococcus species were all l00% resistant to Chloramphenicol and Ampiclox respectively. It is therefore important to adopt appropriate abattoir wastewater treatment measures to prevent the contamination of the environment including surface water and ground water. Implementation of low cost, low technology management practices like separation of solids by screening, blood separation (protein recovery), primary settling, etc should be carried out to reduce the period of delayed degradation.