ABSTRACT

This study evaluated the antibiogram of bacterial isolates from various clinical (urine, high vaginal swab, wound swab, throat swab and stool) samples in Umuahia. In this study, a total of Sixty six (66) bacterial species were isolated and identified using morphological characteristics, pigmentation on media, microscopy, biochemical and sugar fermentation methods. Bacterial species isolated include; Staphylococcus aurlies. Escherichia coli, Shigella species, Pseudomonas species, Streptococcus species, Proteus species and Salmonella species. It was observed in this study that Escherichia co/i and Staphylococcus aurues were the most frequently distributed isolates among the samples with a percentage occurrence of 21(31.8%) and 22(33.3%) respectively. It was also revealed in this study that the antibiotic susceptibility and resistant profile for Gram negative and positive isolates varied from sample to sample as well as isolate to isolate, Escherichia coli and Staphylococcus aurues isolates had high level of susceptibility to Amoxil AML (20rncg), Erythromycin E (30mcg) and Levofloxacin LEV (20mcg) in almost all the clinical specimens. In case of resistant to antibiotics tested in this study, all species isolated showed high level resistance against almost all antibiotics used in this study. It was also noted in this study that Chloramphenicol (6mm), Rifampicin (7mm), Ampiclox (11mm), Amoxil (9mm) and Norfioxacin (8mm) showed high resistance against the various isolates. The antibiogram result showed that all the Gram-negative and positive bacteria isolated in this study were highly sensitive to Levofloxacin (LEX) 20mcg (50%), Ciprofloxacin (CPX) 10mcg (39%), Erythromycin (E) 30mcg (41%), Gentamycin (CN) 10mcg (36%) and Ampiclox (APX) 20mcg (36%). On the other side, the high level of resistance recorded across the Gram Negative and Gram Positive bacteria isolated in this study is worrisome. It could be deduced that the hospital under study could be a potential reservoir of nosocomial pathogens that express different levels of drug resistance factors. However, Levofloxacin (LEX) and Erythromycin (E) antibiotic could be of alternative of choice to use and to control GramNegative and Gram Positive bacterial infection as an effective antibacterial agent as revealed from the present study