ABSTRACT

The antibacterial activity of bile on bacteria isolated from faeces, urine and blood was determined in this study precisely Escherichia coli and Salmonella spp Bile from three animal origin(cow ,goat and chicken) was extracted using alcohol and water and their antibacterial activity tested using the agar well diffusion method at different concentrations . The crude extracts exerted the highest inhibition zone diameter within the range of 20mm-49mm and 38mm-48mm against Salmonella spp and Escherichia coli respectively, followed by the alcoholic extract with inhibition zone diameter in the range of 6mm-35nim and 7mm-33mm against Salmonella spp and Escherichia coli respectively. While the aqueous extract exhibited the least activity in the range of 5mm-25mm and 7mm- 25mm against Salmonella spp and Eseherichia coli respectively. The MIC of the bile extracts which was determined using the tube macrodilution method and the MBC showed that bile have both bactericidal and hacteriostatic activity on the test organisms. The findings of this work has shown that bile contains some substances that can exert inhibitory effect on bacteria hence can be employed in the treatment of diseases caused by these groups of bacteria.