ABSTRACT
Waste
engine oil (WEO) contaminated soil has been considered as harmful to the soil
and plants physiology as well. The study was carried out to evaluate the effect
of waste engine oil contaminated soil on the anatomy of Setaria barbata and Digitaria
horizontalis and the concentrations
were 0%, 2%, 4%,7% and 10% respectively.
The experimental grasses (D.
horizontalis and S. barbata) were
collected from the wild fallow farmland in the university campus and the waste
engine oil obtained as pooled used engine oil from heavy-duty vehicle at motor
mechanic workshop in Umuahia, Abia state. The soil used in
this research were sieved and homogenized with the waste engine oil. The
plants were raised in buckets containing 4kg of sieved sand each at 80ml,
160ml, 280ml and 400ml. The waste engine oil was mixed to the 4kg sand in the
buckets. Each treatment including the control was replicated three times (3x).
The plants were allowed for six weeks. At the control, the stomata of S. barbata and D. horizontalis were clearly viewed but as the concentration
increased reduction in stomata and increase in sinous epidermal cell wall were
seen. Large parenchyma and intercellular air spaces was observed in the stem
then the root experienced distortion in the parenchyma pith on both plants (D. horizontalis and S. barbata). This showed that at high concentration of waste engine
oil contamination, the anatomic structure and arrangement of cells and tissues
are affected negatively and the ability for the two experimental plants (D. horizontalis and S. barbata) to withstand this stress made them fit for
phytoremediation.
TABLE
OF CONTENTS
Title page
i
Declaration ii
Certification iii
Dedication iv
Acknowledgement v
Table of contents vi
List of Figures vii
List of
Plates
viii
Abstract ix
CHAPTER 1
INTRODUCTION
1.1 Background
Information 1
1.2 Justification
of the study 3
1.3 Objectives of
the study 4
CHAPTER 2
LITRATURE REVIEW
2.1. Botany of the experimental plants. 5
2.1.1 Taxonomic classification of Digitaria horizontalis 5
2.1.2 Botany of
Digitaria horizontalis 5
2.1.3 Habitat/Ecology of Digiteria horizontalis 6
2.2.1 Taxonomic Classification of Setaria barbata 8
2.2.2 Botany of Setaria
barbata. 8
2.2.3 Habitat/Ecology of Setaria barbata. 9
2.3 Overview of the
effects of waste engine oil contamination on soil. 10
2.4 Effects of
waste engine oil contaminated soil on the plant anatomy 11
2.5 Effects of waste
engine oil treated soil in plant root anatomy 11
2.6 Review of phytoremediation 12
2.7
Remediation techniques 13
2.7.3 Phytoextraction 15
2.7.4
Phytostabilization 15
2.8 Application of plants for phytoremediation 17
2.9 Safety measures in
phytoremediation process 18
CHAPTER 3
MATERIALS
AND METHOD
3.1 Study area 20
3.2 Experimental
design 20
3.3 Collection of
soil samples 20
3.4 Collection of plant samples 21
3.5 Collection of waste engine oil 21
3.6 Soil treatment 21
3.7 Anatomical Studies 22
3.8 Epidermal Peels 22
3.9
Photomicrography 23
CHAPTER 4
RESULTS 24
CHAPTER 5
5.1
Discussion 36
5.2
Conclusion
37
5.3
Recommendation ` 38
CHUKWU, C (2021). Studies On Anatomy Of Digitaria Horizontalis H. And Setaria Barbata L. As A Phytoremediator Of Waste Engine Oil Contaminated Soil.. Mouau.afribary.org: Retrieved Nov 14, 2024, from https://repository.mouau.edu.ng/work/view/studies-on-anatomy-of-digitaria-horizontalis-h-and-setaria-barbata-l-as-a-phytoremediator-of-waste-engine-oil-contaminated-soil-7-2
CHUKWU, CHUKWU. "Studies On Anatomy Of Digitaria Horizontalis H. And Setaria Barbata L. As A Phytoremediator Of Waste Engine Oil Contaminated Soil." Mouau.afribary.org. Mouau.afribary.org, 08 Jan. 2021, https://repository.mouau.edu.ng/work/view/studies-on-anatomy-of-digitaria-horizontalis-h-and-setaria-barbata-l-as-a-phytoremediator-of-waste-engine-oil-contaminated-soil-7-2. Accessed 14 Nov. 2024.
CHUKWU, CHUKWU. "Studies On Anatomy Of Digitaria Horizontalis H. And Setaria Barbata L. As A Phytoremediator Of Waste Engine Oil Contaminated Soil.". Mouau.afribary.org, Mouau.afribary.org, 08 Jan. 2021. Web. 14 Nov. 2024. < https://repository.mouau.edu.ng/work/view/studies-on-anatomy-of-digitaria-horizontalis-h-and-setaria-barbata-l-as-a-phytoremediator-of-waste-engine-oil-contaminated-soil-7-2 >.
CHUKWU, CHUKWU. "Studies On Anatomy Of Digitaria Horizontalis H. And Setaria Barbata L. As A Phytoremediator Of Waste Engine Oil Contaminated Soil." Mouau.afribary.org (2021). Accessed 14 Nov. 2024. https://repository.mouau.edu.ng/work/view/studies-on-anatomy-of-digitaria-horizontalis-h-and-setaria-barbata-l-as-a-phytoremediator-of-waste-engine-oil-contaminated-soil-7-2