Prevalence And Antibiogram Of E.Coli Isolates From Bovine Gastro-Intestinal Tracts From Ubakala Ultramodern Abattoir, Umuahia Abia State
ABSTRACT
The study was carried out to investigate the prevalence of E coli in gastro-intestinal tracts of slaughtered cattle in Ubakala Ultramodern abattoir, Umuahia in Abia state. A total of 150 samples were collected (using sterile swab stick) from different segments (abomasums, duodenum, jejunum, ileum, colon and caecum) of the gastro-intestinal tract of slaughtered cattle, over a period of one month. The samples were cultured on Mackonkey Agar and Eosin Methylene Blue Agar for the detection of E coli and the isolates were further subjected to the following tests; indole test, methyl red test, voges proskauer test, citrate test, glucose, lactose and mannitol fermentation tests. Antibiotic sensitivity test was carried out using the Disc Diffusion Method. A total of 55 E.coli isolates were obtained which gave a total prevalence of 36.6% and this could be attributed to poor or improper hygiene, being practiced by abattoir workers on a daily basis. The antibiotic optudisc, was used in the antibiotic sensitivity test and is made up of ten (10) antibiotic drugs namely; Tarivid (OFX) 10mcg, Reflacin(PEF) 10mcg, Ciproflox(CPX) 10mcg, Augmentin(AU) 30mcg, Gentamycin(CN) 10mcg, Streptomycin(S)30mcg, Ceprorex(CEP)10mcg, Nalidixic Acid(NA)30mcg, Septrin(SXT)30mcg and Amplicin(PN)30mcg. Majority of the E coli isolates were found to be sensitive to; Tarivid(OFX) 10mcg, Septrin(SXT)30mcg, Ciproflox(CPX)10mcg, Reflacin(PEF)10mcg and Gentamycin(CN)10mcg.This study evidenced a considerable presence of E coli in the duodenum, jejunum, ileum and colon of slaughtered cattle.
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UZOECHI, E (2021). Prevalence And Antibiogram Of E.Coli Isolates From Bovine Gastro-Intestinal Tracts From Ubakala Ultramodern Abattoir, Umuahia Abia State. Mouau.afribary.org: Retrieved Nov 23, 2024, from https://repository.mouau.edu.ng/work/view/prevalence-and-antibiogram-of-ecoli-isolates-from-bovine-gastro-intestinal-tracts-from-ubakala-ultramodern-abattoir-umuahia-abia-state-7-2
ERIC-CLEVER, UZOECHI. "Prevalence And Antibiogram Of E.Coli Isolates From Bovine Gastro-Intestinal Tracts From Ubakala Ultramodern Abattoir, Umuahia Abia State" Mouau.afribary.org. Mouau.afribary.org, 03 Sep. 2021, https://repository.mouau.edu.ng/work/view/prevalence-and-antibiogram-of-ecoli-isolates-from-bovine-gastro-intestinal-tracts-from-ubakala-ultramodern-abattoir-umuahia-abia-state-7-2. Accessed 23 Nov. 2024.
ERIC-CLEVER, UZOECHI. "Prevalence And Antibiogram Of E.Coli Isolates From Bovine Gastro-Intestinal Tracts From Ubakala Ultramodern Abattoir, Umuahia Abia State". Mouau.afribary.org, Mouau.afribary.org, 03 Sep. 2021. Web. 23 Nov. 2024. < https://repository.mouau.edu.ng/work/view/prevalence-and-antibiogram-of-ecoli-isolates-from-bovine-gastro-intestinal-tracts-from-ubakala-ultramodern-abattoir-umuahia-abia-state-7-2 >.
ERIC-CLEVER, UZOECHI. "Prevalence And Antibiogram Of E.Coli Isolates From Bovine Gastro-Intestinal Tracts From Ubakala Ultramodern Abattoir, Umuahia Abia State" Mouau.afribary.org (2021). Accessed 23 Nov. 2024. https://repository.mouau.edu.ng/work/view/prevalence-and-antibiogram-of-ecoli-isolates-from-bovine-gastro-intestinal-tracts-from-ubakala-ultramodern-abattoir-umuahia-abia-state-7-2