ABSTRACT
Milk is an important source of nutrient to humans and animals. It is meant to be the first and the only food for the offspring of mammals as it is capable to serve as food. This study evaluated the microbial quality of raw milk from dairy farm. About three raw milk samples were collected from Fulani cattle settlement, Umuahia, Abia State. The samples were analyzed using the microbiological techniques for isolation and identification of isolates. The microbial load of the samples ranged from 8.2x105 CFU/ml to 6.0 x 105 CFU/ml. About five (5) bacterial genera were isolated which comprised of 3 (25%) Lactobacillus spp., 3 (25%) Listeria spp., 3 (25%) Salmonella spp., 2 (16.7%) Proteus spp. And 1 (8.3%) The presence of bacteria responsible for intoxication such as Staphylococcus aureus may be a public health problem if no precaution is taken against this contamination.
TABLE OF CONTENTS
Title page i
Certification ii
Dedication iii
Acknowledgment iv
Table of contents v
List of tables vii
Abstract viii
CHAPTER ONE
1.0 Introduction 1
1.1 Aims and objectives of the study 5
CHAPTER TWO
2.0 Literature review 6
2.1 Definition and composition of milk 6
2.2 General overview of milk production and safety in a dairy farm 6
2.3 Source of microbial contamination of milk 7
2.4 Milkborne infections and pathogenic micro-organisms 8
2.5 Hygiene, handling and microbial evaluation of raw milk 8
2.6 Prevention and control of microbial contamination of milk 9
2.7 Antimicrobial residues, bacterial resistance and their effects
to animals and humans 9
2.8 Milk-borne infections and pathogenic microorganisms 11
2.9 Nigerian policy on food hygiene and safety 13
2.9.1 National legislations 13
2.10 Dairy development in Nigeria 14
CHAPTER THREE
3.0 Materials and methods 16
3.1 Study area and sample collection 16
3.2 Sampling of milk/size 16
3.3 Milk sample handling 16
3.4 Media preparation 16
3.5 Inoculation and incubation of the sample 17
3.6 Identification and characterization of isolates 17
3.6.1 Gram staining technique 17
3.7 Biochemical tests 18
3.7.1 Catalase test 18
3.7.2 Oxidase test 18
3.7.3 Indole test 18
3.7.4 Citrate utilization test 19
3.7.5 Motility test 19
3.7.6 Coagulase test 19
CHAPTER FOUR
4.0 Results 20
CHAPTER FIVE
5.0 Discussion 24
5.1 Conclusion 25
5.2 Recommendation 26
REFERENCES
PRECIOUS, M (2020). Microbial Evaluation Of Raw Milk From Dairy Farm. Mouau.afribary.org: Retrieved Nov 14, 2024, from https://repository.mouau.edu.ng/work/view/microbial-evaluation-of-raw-milk-from-dairy-farm
MOUAU/12/23224, PRECIOUS. "Microbial Evaluation Of Raw Milk From Dairy Farm" Mouau.afribary.org. Mouau.afribary.org, 04 Jun. 2020, https://repository.mouau.edu.ng/work/view/microbial-evaluation-of-raw-milk-from-dairy-farm. Accessed 14 Nov. 2024.
MOUAU/12/23224, PRECIOUS. "Microbial Evaluation Of Raw Milk From Dairy Farm". Mouau.afribary.org, Mouau.afribary.org, 04 Jun. 2020. Web. 14 Nov. 2024. < https://repository.mouau.edu.ng/work/view/microbial-evaluation-of-raw-milk-from-dairy-farm >.
MOUAU/12/23224, PRECIOUS. "Microbial Evaluation Of Raw Milk From Dairy Farm" Mouau.afribary.org (2020). Accessed 14 Nov. 2024. https://repository.mouau.edu.ng/work/view/microbial-evaluation-of-raw-milk-from-dairy-farm