ABSTRACT
The plant Stachytarpheta
angustfolia is a medicinal plant which comes from the genus Stachytarpheta of
the family Verbenaceae. This study is aimed at isolating the plant extract,
characterizing it and determining the phytochernical and analgesic properties
of the plant. Extraction was done using chloroform and column chromatography
was used for the elution of the crude extract using silica gel as the
stationary phase. Solvents used as the mobile phase were petroleum ether,
chloroform and methanol. These solvents had differing polarity; from non-polar
to polar. A total of 26 fractions were collected after elution. Purity of the
fl-actions were monitored using thin layer chromatography (TLC) on silica gel
coated plates. Solvents used were petroleum ether, choroform and methanol.
Quantitative and qualitative screening test were both carried out on the crude extract
to determine the phytochemical properties of the plant. Analgesic analysis was
carried out using acetic-acid writhing reflex method. After TLC, fraction 26 yielded
a single spot with the solvents, petroleum ether, chloroform and methanol in
the ration 1:3:6 (R =0.55). The structure was elucidated using FTIR, 'HNMR,
13CNMR, DEPT-135 and C-H COSY and the structure had a molecular formula of
C28H4803. The compound was characterized as a triterpenoid. Phytochemical
screening using the standard qualitative techniques revealed the presence of
flavonoids, phenolics, tannins, steroids and terpenoids. The result showed very
strong presence of Alkaloids, Flavonoids and terpenoids. The crude extract also
showed good analgesic property. As a follow-up on the study, it is anticipated
that the compound can further be explored to reveal its potential applications.
CHIKEZIE, U (2021). Isolation, Characterization And Analgesic Properties Of Chloroform Extract From Stachytarpheta Angustifolia. Mouau.afribary.org: Retrieved Dec 25, 2024, from https://repository.mouau.edu.ng/work/view/isolation-characterization-and-analgesic-properties-of-chloroform-extract-from-stachytarpheta-angustifolia-7-2
UZOAMAKA, CHIKEZIE. "Isolation, Characterization And Analgesic Properties Of Chloroform Extract From Stachytarpheta Angustifolia" Mouau.afribary.org. Mouau.afribary.org, 05 Jul. 2021, https://repository.mouau.edu.ng/work/view/isolation-characterization-and-analgesic-properties-of-chloroform-extract-from-stachytarpheta-angustifolia-7-2. Accessed 25 Dec. 2024.
UZOAMAKA, CHIKEZIE. "Isolation, Characterization And Analgesic Properties Of Chloroform Extract From Stachytarpheta Angustifolia". Mouau.afribary.org, Mouau.afribary.org, 05 Jul. 2021. Web. 25 Dec. 2024. < https://repository.mouau.edu.ng/work/view/isolation-characterization-and-analgesic-properties-of-chloroform-extract-from-stachytarpheta-angustifolia-7-2 >.
UZOAMAKA, CHIKEZIE. "Isolation, Characterization And Analgesic Properties Of Chloroform Extract From Stachytarpheta Angustifolia" Mouau.afribary.org (2021). Accessed 25 Dec. 2024. https://repository.mouau.edu.ng/work/view/isolation-characterization-and-analgesic-properties-of-chloroform-extract-from-stachytarpheta-angustifolia-7-2