ABSTRACT

The investigation was carried out to isolate antibiotics producing microorganisms around fish pond environment in Umudike. Soil samples were collected from 20 different locations of the fish pond for the isolation of potential antibiotic producing microorganisms. Spread plate technique was used for isolation and screening of microorganism. The media used for the isolation were Sabouraud dextrose agar, Tryptone soy agar and Nutrient agar. Sensitivity test using Staphylococcus aureus, Enterococcus feacalis, Pseudomonas aeruginosa, E.coli, as test organisms was employed  to investigate the antimicrobial activity of the isolates .Out of forty eight microorganisms  isolated, four of the isolates showed evidence of antimicrobial property against any of the bacteria test organisms and  they include; FPC1 which after biochemical screening was identified to be a Streptomyces species.,., FPA2 a Bacillus species., FPD2 a Bacillus species., and FPF3 a  Clostridium species., all the microbial isolates were inhibitory to the growth of atleast one of  the test bacteria . FPC1 produce inhibition zone of 9mm against P. areuginosa ,FPA2 produce inhibition zone of 8mm against  staphylococcus aureus ,  FPD2 produce inhibiton zone of 7mm  against E.faecalis and FPF3 produce inhibition of 6mm against P.aeruginosa. The result of this investigation demonstrated that strains of antibiotics producing microorganism are present in soil around fish pond environment  in  Umudike  and could be harnessed by pharmaceutical industries for the production of antibiotic from local sources. 

TABLE OF CONTENTS

CERTIFICATION i                    

DEDICATION                                                                                                              ii

ACKNOWLEDGEMENTS                                                                                          iii

TABLE OF CONTENTS                                                                                              iv

LIST OF TABLES                                                                                                        vi

ABSTRACT                                                                                                                  vii

CHAPTER ONE

1.0 INTRODUCTION 1

1.1 Aims and Objectives                                                                                4

CHAPTER TWO

2.0 Literature Review 5       

2.1 Factors affecting antibiotics production by microorganism                          7

2.2 Past advances made in search for antibiotics producing 

              microorganisms in novel  environment                                                        11 

2.3 Techniques employed in screening for antibiotics producing

             Microorganism 15

CHAPTER THREE

Materials and Method 18

3.0 Sample Collection and Preparation 18                                                            

3.1 Media Preparation 18

3.2 Sample Preparation 18

3.3 Inoculation 19

3.4 Sub Culturing 19

3.5 Gram Staining 19

3.6 Biochemical Test 20

3.6.1 Hydrogen Sulfide test 20

3.6.2 Catalase Test 20

3.6.3 Starch Hydrolysis Test 20

3.7 Screening for Antimicrobial Activity 20

CHAPTER FOUR

4.1 RESULT 22

CHAPTER FIVE

5.0 Discussion 26

5.1 Conclusion 27

5.2 Recommendation 28

 REFRENCE