ABSTRACT

This project involved isolation and identification of microalgae from pond and fresh water. The aim of the research was to enumerate the algal species that inhabit pond and fresh water environments. Water samples were collected at one week interval for a period of four weeks. Standard Microbiological procedures were employed in the culturing of the algal species. F2 medium was used for the culturing. The algal colonies obtained were subjected to microscopic and morphology identification. Five species of algae (Cyclotella bodanica Eulenst, Cyclotella meneghiniana, Diatomelongatum agardh, Rhizosolenia longiseta Zach, Melosira Varians) were identified. The research concluded that microalgae inhabit aquatic environment such as pond and might have some useful activities they play on the aquatic environment.

TABLE OF CONTENTS

Certification………………………………………………………………………………....i

Dedication………………………………………………………………………………….ii

Acknowledgement…….……………………………………………………………...……iii

Table of Contents………………………...………………………………………………..iv

List of Tables……………...………………………………………………………………..v

Abstract............................………………………………………………………………….vi

Chapter One…………………………………………………………….....……………...1

Introduction………………………………………………………………………...…......1 

1.1 Background of the Study………………………………………………….………..1

1.2 Microalgae…………………………………………………………………………2

1.3 Objective of the Study……………………………………………………...……...5

Chapter Two…………………………………………………………………………..…..6

 2.1 Literature Review………………………….……………………………………….6

2.2.1 Microalgae Culturing (Cultivation)………………………………………………...6

2.2.2 Microalgae Growth and Cultivation………………………………………………..8

Chapter Three………………………………………………………………………….. 11

3.0 Material and Methods………………………….………………………………….11

3.1 Field Collection…………………………………………………………………...11

3.2 Culture Medium/Nutrient…………………………………………………………11

3.2.1 Media Preparation………………………………………………………………...12

3.2.2 Materials…………………………………………………………………………..12

3.3 Methods…………………………………………………………………………...12

3.3.1 Sterilizing Culture Medium……………………………………………………….13

3.4 Isolation………………………………………………………………………...…14

3.5 Morphological Identification……………………………………………………...15

Chapter Four………………………………………………………………………..……16

4.1 Result……………………………………………………………………..……….16

Chapter Five………………………………………………………...……………………18

5.1 Discussion………………………………………………………………………...18

5.2 Conclusion and Recommendation………………………………………………..18

References