ABSTRACT

Chemical investigation of the bioactive constituents in Stachytarpheta jamaicensis resulted in the isolation of four new steroidal glucosides from the leaves and three f NMR, IR and Mass Spectra were used to propose the structures of the bioactive compounds isolated from Stachytarpheta jamaicensis. Ethanol (98%) extract of Stachytarpheta jamaicensis exhibited significant in-vitro antimicrobial activity against Staphylococcus aureus, Streptococcusfecalis, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. The minimum inhibitory concentration (MIC) was attained at 6.25-12.5mg/ml for the leaves and 12.5-25mg/ml for the stem barks. The phytochemical evaluation revealed the availability of bioactive compounds comprising alkaloids (3.048-8.265mg/100g), tannins (0.080-0.254mg/100g), phenols (0.014-0.029mg/100g), saponins (0.564-0.744mg/l 00g), flavonoids (0.780- 9.820mg/100g) and oxalates (0.667-1.277mg/ 100g). The minerals content revealed magnesium (1.094-I.277mg/100g), calcium (3.707-4.509mg/100g), potassium (1.2- 1.3mg/100g), sodium (0.325-0.55mg/100g), phosphorous (0.689-0.708mg/100g), manganese (5.80-7.98mg/I00g), zinc (11.13-15. lOmg/lOOg), copper (4.88- 4.98mg/100g), lead (1.60-2.46mg/100g) and iron (51.30-53.08mg/100g). The vitamins content was ascorbic acid (92.40-57.20mg/100g), 0-carotenoid (312.50- 474.54mg/100g), riboflavin (2.63-4.13mg/l00g), thiamin (0.38-0.75mg/100g) and niacin (0.84-1.4 lmg/100g). The presence of steroids and triterpenoids in Stachytarphetajamaicensis shows that the herb is of potential phytotherapeutic value in the treatment of diseases and hormone replacement therapies. Stachytarpheta jamaicensis offers a wide scope for pharmaceutical industries to use in drug formulation. new triterpenoids from the stem barks of Stachytarphetajamaicensis. 'H NMR, 13C