Isolation And Antibiotic Sensitivity Test Of Pathogenic Bacteria From Ready-To-Eat Food Sold In Michael Okpara University Of Agriculture, Umudike
ABSTRACT
Today the issue of food safety is a global problem that gets main concern in setting public health policy. The eruption of diseases caused by food contamination occurs in places where sanitation and hygiene conditions are generally poor. This study was conducted to isolate and determine the antibiogram of bacteria pathogens in ready-to-eat foods sold in MOUAU campus. Four ready-to-eat food samples were collected from different vendors within the campus and analyzed immediately through serial dilution, inoculation, incubation, subculture, microscopy and biochemical to confirm the isolated organisms. Antibiotics sensitivity test was also carried out to determine antibiotic sensitivity on the isolated organisms. A total of four pathogenic bacteria were isolated: Staphylococcus aureus (40%), Salmonella (17.5%), Escherichia coli (30%) and Shigella (12.5%). Eight different antibiotic were used to carry out antibiogram of the isolates as the inhibition zone was measured in millimeter: Oflovacin (OFX), Amoxil (AMX), Gentamycin (CN), Augmentin (AUG), Ciprofloxacin (CPX), Chloramphenicol (CH), Ampiclox (APX) and Streptomycin (S). (AUG) and (CPX) had high effect on Salmonella and Escherichia coli and was less on Staphylococcus aureus while (OFX) and (S) were very effective on Staphylococcus aureus and less effective on Escherichia coli and Salmonella. This shows the poor hygiene level of the food vendors as majority of the food vendors lacks western education. These vendors have to receive education and training on food hygiene to improve the safety of foods in MOUAU and thereby heighten the safety of consumers.
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EBUKA, P (2021). Isolation And Antibiotic Sensitivity Test Of Pathogenic Bacteria From Ready-To-Eat Food Sold In Michael Okpara University Of Agriculture, Umudike. Mouau.afribary.org: Retrieved Nov 15, 2024, from https://repository.mouau.edu.ng/work/view/isolation-and-antibiotic-sensitivity-test-of-pathogenic-bacteria-from-ready-to-eat-food-sold-in-michael-okpara-university-of-agriculture-umudike-7-2
PROMISE, EBUKA. "Isolation And Antibiotic Sensitivity Test Of Pathogenic Bacteria From Ready-To-Eat Food Sold In Michael Okpara University Of Agriculture, Umudike" Mouau.afribary.org. Mouau.afribary.org, 04 Nov. 2021, https://repository.mouau.edu.ng/work/view/isolation-and-antibiotic-sensitivity-test-of-pathogenic-bacteria-from-ready-to-eat-food-sold-in-michael-okpara-university-of-agriculture-umudike-7-2. Accessed 15 Nov. 2024.
PROMISE, EBUKA. "Isolation And Antibiotic Sensitivity Test Of Pathogenic Bacteria From Ready-To-Eat Food Sold In Michael Okpara University Of Agriculture, Umudike". Mouau.afribary.org, Mouau.afribary.org, 04 Nov. 2021. Web. 15 Nov. 2024. < https://repository.mouau.edu.ng/work/view/isolation-and-antibiotic-sensitivity-test-of-pathogenic-bacteria-from-ready-to-eat-food-sold-in-michael-okpara-university-of-agriculture-umudike-7-2 >.
PROMISE, EBUKA. "Isolation And Antibiotic Sensitivity Test Of Pathogenic Bacteria From Ready-To-Eat Food Sold In Michael Okpara University Of Agriculture, Umudike" Mouau.afribary.org (2021). Accessed 15 Nov. 2024. https://repository.mouau.edu.ng/work/view/isolation-and-antibiotic-sensitivity-test-of-pathogenic-bacteria-from-ready-to-eat-food-sold-in-michael-okpara-university-of-agriculture-umudike-7-2