ABSTRACT
Green leafy vegetables are
indispensable in human diets, especially in Africa, providing essential
nutrients such as vitamins, minerals, and fibers which help in combating
anaemia and other nutritional defieciencies. However, underutilized vegetables
like Piper methysticum remain underexplored despite their potential
nutritional and therapeutic benefits. This study aimed at evaluating
the effects of aqueous extracts of Piper
methysticum on some haematological parameters in 2,4-dinitrophenylhydrazine
anaemic rats. The P. methysticum leaves
were turned to extract and the chemical contents were analyzed using standard
laboratory procedures. The results of the proximate showed that the leaves had
moisture content 9.62%, fat content 1.07%,
crude fibre 8.67%, crude protein 14.94% and
carbohydrate 55.43%. The results of the mineral evaluation showed that the
leaves had Fe (5.84 mg/100g), Zn (3.64 mg/100g), Cu (0.79
mg/100g) and Mn (0.47 mg/100g). Rats were made
anaemic with phenylhydrazine administered at 40mg/kg body weight, grouped and
labelled Group 1, Group 2, Group 3 and Group 4 respectively with Group 1
(normal rats: not induced) serving as the positive control, Group 2 (anaemia
induced: no treatment) serving as the negative control. Groups 3 and 4 were
treated with low dose extract (150mg/kg body weight) and high dose extract
(300mg/kg body weight). After 15 consecutive days of treatment, blood samples
were collected from the rats and used to evaluate the effects of the extracts
on their PCV, RBC and haemoglobin. The data generated were statistically
analysed using One Way Analysis of Variance (ANOVA) and Paired T-test. The
results of the RBC, PCV and haemoglobin analysis showed that the Group 1 had
the highest values at 7.01
-- (2025). Haematological Effects Of Aqueous Awa (Piper Methysticum) Leaf Extract In 2,4-Dinitrophenylhydrazine-Induced Anaemic Rats:-Abel, Glory C. Mouau.afribary.org: Retrieved Jul 09, 2025, from https://repository.mouau.edu.ng/work/view/haematological-effects-of-aqueous-awa-piper-methysticum-leaf-extract-in-24-dinitrophenylhydrazine-induced-anaemic-rats-abel-glory-c-7-2
--. "Haematological Effects Of Aqueous Awa (Piper Methysticum) Leaf Extract In 2,4-Dinitrophenylhydrazine-Induced Anaemic Rats:-Abel, Glory C" Mouau.afribary.org. Mouau.afribary.org, 08 Jul. 2025, https://repository.mouau.edu.ng/work/view/haematological-effects-of-aqueous-awa-piper-methysticum-leaf-extract-in-24-dinitrophenylhydrazine-induced-anaemic-rats-abel-glory-c-7-2. Accessed 09 Jul. 2025.
--. "Haematological Effects Of Aqueous Awa (Piper Methysticum) Leaf Extract In 2,4-Dinitrophenylhydrazine-Induced Anaemic Rats:-Abel, Glory C". Mouau.afribary.org, Mouau.afribary.org, 08 Jul. 2025. Web. 09 Jul. 2025. < https://repository.mouau.edu.ng/work/view/haematological-effects-of-aqueous-awa-piper-methysticum-leaf-extract-in-24-dinitrophenylhydrazine-induced-anaemic-rats-abel-glory-c-7-2 >.
--. "Haematological Effects Of Aqueous Awa (Piper Methysticum) Leaf Extract In 2,4-Dinitrophenylhydrazine-Induced Anaemic Rats:-Abel, Glory C" Mouau.afribary.org (2025). Accessed 09 Jul. 2025. https://repository.mouau.edu.ng/work/view/haematological-effects-of-aqueous-awa-piper-methysticum-leaf-extract-in-24-dinitrophenylhydrazine-induced-anaemic-rats-abel-glory-c-7-2