ABSTRACT
Tomato is one of the most popular
consumed vegetable crops worldwide mainly as fresh or processed products. The
antimicrobial potentials of lactic acid bacteria metabolites against tomato
paste spoilage microorganisms were evaluated.
Cow milk and Tomato samples were purchased. The tomato samples were processed into tomato paste. Standard
microbiological methods were used in isolation, microbial count and
identification of organisms. Cow milk samples were used for the isolation of
lactic acid bacteria. Antimicrobial effects of the isolated lactic acid bacteria metabolites were carried
out on the spoilage isolates from tomato paste samples using the agar well diffusion method, while shelf-life study was
done on the tomato paste samples using the lactic acid bacteria metabolites as
biopreservative agents for a period of 14 days. The results showed that total
viable count ranged from 2.81 x
107Cfu/ml - 1.3 x 106Cfu/ml, the coliform count from 6.5 x 104Cfu/ml – 1.1
x 104Cfu/ml, while the fungal count was from 3.0 x 104Cfu/ml
– 1.5 x 104Cfu/ml. The
bacterial isolates from the tomato samples were Bacillus sp and Pseudomonas sp, while the fungal isolates were Rhizopus sp, Saccharomyces sp, Fusarium
sp, Apergillus sp and Mucor sp. Lactic acid bacteria isolates from the cow milk samples were Lactobacillus fermentum M6, Lactobacillus fermentum LF-12 and Lactobacillus fermentum M8.The strains
of Lactobacillus fermentum were able
to grow at different temperature, pH and NaCl concentration and also produced
antimicrobial compounds with Lactobacillus
fermentum LF-12 recording the highest lactic acid (0.98 g/l), diacetyl
(1.77 g/l) and hydrogen perioxide (1.60 g/l). The metabolites from strains of Lactobacillus fermentum were all potent
against the spoilage microorganisms with zones of inhibition ranging from 2 mm
to 12 mm. There were significant differences (p<0.005) in the activities of
the metabolites of the strains of Lactobacillus
fermentum. The result of the shelf-life study revealed a great reduction in
bacterial load and growth over a period of 14 days of storage. This shows that
strains of Lactobacillus fermentum
can be used as bioperservative agents
in preserving tomato paste as an alternative to chemical preservatives.
CHIMEREZE, C (2023). Evaluation Of The Antimicrobial Potentials Of Lactic Acid Bacteria Metabolites Against Tomato Paste Spoilage Microorganisms . Mouau.afribary.org: Retrieved Nov 12, 2024, from https://repository.mouau.edu.ng/work/view/evaluation-of-the-antimicrobial-potentials-of-lactic-acid-bacteria-metabolites-against-tomato-paste-spoilage-microorganisms-7-2
CHIMEREZE, CHIMEREZE. "Evaluation Of The Antimicrobial Potentials Of Lactic Acid Bacteria Metabolites Against Tomato Paste Spoilage Microorganisms " Mouau.afribary.org. Mouau.afribary.org, 27 Mar. 2023, https://repository.mouau.edu.ng/work/view/evaluation-of-the-antimicrobial-potentials-of-lactic-acid-bacteria-metabolites-against-tomato-paste-spoilage-microorganisms-7-2. Accessed 12 Nov. 2024.
CHIMEREZE, CHIMEREZE. "Evaluation Of The Antimicrobial Potentials Of Lactic Acid Bacteria Metabolites Against Tomato Paste Spoilage Microorganisms ". Mouau.afribary.org, Mouau.afribary.org, 27 Mar. 2023. Web. 12 Nov. 2024. < https://repository.mouau.edu.ng/work/view/evaluation-of-the-antimicrobial-potentials-of-lactic-acid-bacteria-metabolites-against-tomato-paste-spoilage-microorganisms-7-2 >.
CHIMEREZE, CHIMEREZE. "Evaluation Of The Antimicrobial Potentials Of Lactic Acid Bacteria Metabolites Against Tomato Paste Spoilage Microorganisms " Mouau.afribary.org (2023). Accessed 12 Nov. 2024. https://repository.mouau.edu.ng/work/view/evaluation-of-the-antimicrobial-potentials-of-lactic-acid-bacteria-metabolites-against-tomato-paste-spoilage-microorganisms-7-2