COMPARISON OF THE IN/VITRO SENSITIVITY TESTING OF AMINOGLYCOSIDES AGAINST FLUOROQUINOLONE AGENTS IN URINARY TRACT INFECTION ISOLATES

MUNACHIMSO GOODLUCK MOUAU/11/18576 | 51 pages (10811 words) | Projects
Microbiology | Co Authors: ODOZIE

ABSTRACT

In this study a total number of 70 samples were collected from different individuals while 34(48.5%) urine samples were from males, 36(51.4%) were females. 32(45.7%) were found positive for urinary tract infections. The most prevalent isolate was Escherichia coli (75%), followed by Proteus mirabilis (12.5%), and the least isolates were Klebsiella pneumoniae (6.2%) and Staphylococcus saprophyticus(6.2%). The urine samples were cultured using cysteine lactose electrolyte deficient medium (CLED), Blood agar and MacConkey agar. Biochemical tests and gram staining were done to identify the isolates. Antimicrobial sensitivity testing was carried out by disc diffusion technique using antibiotic agents such as aminoglycosides (Gentamicin and Streptomycin) and fluoroquinolones (Ofloxacin and Ciprofloxacin) repectively, to determine the invitro sensitivity pattern of the isolates. The results obtained shows that among the isolates tested, maximum susceptibility was found for fluoroquinolone agents than those of aminoglycosides.


TABLE OF CONTENTS                                                                                         PAGES

Title page    i     

Certification    ii

Dedication    iii

Acknowledgement    iv

Table contents     v

List of table                                                                                                                   viii

List of figures                                                                                                                 ix

Abstract      x


CHAPTER ONE

1.0 Introduction      1

1.1 Aim and Objectives      4


CHAPTER TWO

2.0 Literature Review     5

2.1 UTI in Pregnancy                                                                                               8

2.2 Hospital Acquired UTI                                                                                      10

2.3 UTI in Geriatric Patient                                                                                     11

2.4 Isolates                                                                                                               11

2.5 Antimicrobial resistance among UTI pathogens                                               12

2.6 UTI Therapy                                                                                                      14

2.6.1 Fluoroquinolones                                                                                               14

2.6.1.1 General Structure    15

2.6.1.2 Drug-Drug Resistance                                                                                       15

2.6.1.3 Mechanism of Resistance    16

2.6.1.4 Effects of Quinolones on Bacteria                                                                     16

2.6.1.5 Use of Quinolone in the Treatment of UTI                                                       17

2.6.2 Aminoglycosides                                                                                               18

2.6.2.1 Mechanism of action                                                                                         18

2.6.2.2 Route of Administration                                                                                    19 

2.6.2.3 Use of Aminoglycosides in the Treatment UTI                                                19


CHAPTER THREE

3.0 Materials and Methods                                                                                       21

3.1 Study Area                                                                                                          21                                                                      

3.2 Sample Collection                                                                                               21

3.3 Sterilization of Media and Materials      21

3.4 Inoculation of Urine Sample and Isolation of Bacterial Isolates      22

3.5 Microscopy      22

3.6 Identification and Characterization of Isolates                                   23

3.7 Antibiotic Sensitivity Testing                                   23

3.8 Biochemical Test      23

3.8.1 Catalase Tests                               23

3.8.2 Coagulase Test                                                                                                     24

3.8.3 Citrate Test                                      24

3.8.4 Indole Test       25

3.8.5 Methyl red Test          25

3.8.6 Voges – Proskauer Test           25


CHAPTER FOUR

4.0 Results        26


CHAPTER FIVE

5.0 Discussion                         34

5.1 Recommendation           36

5.2 Conclusion        37

References                                                                                     38


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APA

MUNACHIMSO, M (2020). COMPARISON OF THE IN/VITRO SENSITIVITY TESTING OF AMINOGLYCOSIDES AGAINST FLUOROQUINOLONE AGENTS IN URINARY TRACT INFECTION ISOLATES. Mouau.afribary.org: Retrieved Nov 13, 2024, from https://repository.mouau.edu.ng/work/view/comparison-of-the-in-vitro-sensitivity-testing-of-aminoglycosides-against-fluoroquinolone-agents-in-urinary-tract-infection-isolates

MLA 8th

MOUAU/11/18576, MUNACHIMSO. "COMPARISON OF THE IN/VITRO SENSITIVITY TESTING OF AMINOGLYCOSIDES AGAINST FLUOROQUINOLONE AGENTS IN URINARY TRACT INFECTION ISOLATES" Mouau.afribary.org. Mouau.afribary.org, 28 Apr. 2020, https://repository.mouau.edu.ng/work/view/comparison-of-the-in-vitro-sensitivity-testing-of-aminoglycosides-against-fluoroquinolone-agents-in-urinary-tract-infection-isolates. Accessed 13 Nov. 2024.

MLA7

MOUAU/11/18576, MUNACHIMSO. "COMPARISON OF THE IN/VITRO SENSITIVITY TESTING OF AMINOGLYCOSIDES AGAINST FLUOROQUINOLONE AGENTS IN URINARY TRACT INFECTION ISOLATES". Mouau.afribary.org, Mouau.afribary.org, 28 Apr. 2020. Web. 13 Nov. 2024. < https://repository.mouau.edu.ng/work/view/comparison-of-the-in-vitro-sensitivity-testing-of-aminoglycosides-against-fluoroquinolone-agents-in-urinary-tract-infection-isolates >.

Chicago

MOUAU/11/18576, MUNACHIMSO. "COMPARISON OF THE IN/VITRO SENSITIVITY TESTING OF AMINOGLYCOSIDES AGAINST FLUOROQUINOLONE AGENTS IN URINARY TRACT INFECTION ISOLATES" Mouau.afribary.org (2020). Accessed 13 Nov. 2024. https://repository.mouau.edu.ng/work/view/comparison-of-the-in-vitro-sensitivity-testing-of-aminoglycosides-against-fluoroquinolone-agents-in-urinary-tract-infection-isolates

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