ABSTRACT
Gram negative bacteria are the most prevalent cause of urinary tract infection when compared to gram positive bacteria. The gram negative organisms commonly implicated are E. coli, Klebsilla spp, Staphylococcus aureus, protens spp., Psendomonas asmginosa and other gram negative bacteria. Urinary tract infection is most common among females than males and this could be explained by the short urethra of the females which make them prone to urinary tract infection. Mid stream urine samples were collected from UTI patients around MOUAU. Each specimen was cultured using standard bacteriological methods identification of gram negative organisms were made and antibiotic sensitivity testing of the gram negative isolates was carried out. A total of 30 gram negative isolates were used in the study out of which 10 (33.3%) were E. coli, 9(30%) Protens spp 6(20%) pseudomonad aeruginosa and 5(16.7%) klebsiella spp. Nitrofurantoin was the most effective antibiotic against the isolates while the isolates were highly resistant to cefixime and augmentin. E. coli was the most isolated gram negative organism isolated in thus study. Education on the importance of proper treatment of UTI to the general public cannot be over emphasized.
TABLE OF CONTENTS
Cover page i
Title page ii
Certification iii
Dedication iv
Acknowledgement v
Table of contents vi
List of tables viii
List of figures ix
Abstract x
CHAPTER ONE
1.1 Introduction 1
CHAPTER TWO
LITERATURE REVIEW
2.1 Antimicrobial Agents 3
2.2 Penicillin 3
2.2.1 Tetracycline 4
2.2.2 Chloramphenicol 4
2.3 Antibiotic Resistance 5
2.4 Mode of Action of Antibiotics 6
2.5 Gram Negative Bacteria 8
2.5.1. Escherichia coli 8
2.5.2. Klebsiella Specie 8
2.5.3 Proteus Species 9
2.5.4. Pseudomonas Species. 9
2.6. Urinary Tract Infections 9
2.6.1. Types of Urinary Tract Infections 10
CHAPTER THREE
MATERIALS AND METHODS
3.1 Sample Collections 11
3.2 Sterilization of Materials 11
3.3 Isolation of bacteria from urine Samples 11
3.4 Grem Stain Test 11
3.4.1 Biochemical tests 12
3.4.2 Catalase test 12
3.4.3 Peptone Water Sugar Test 12
3.4.4 Antibiotic Sensitivity Testing 13
CHAPTER FOUR
4.0 Results 14
CHAPTER FIVE
5.0 Discussions 19
5.1 Conclusion 20
REFERENCE
APPENDIX 1
APPENDIX 2
IHUOMA, M (2020). ANTIMICROBIAL SUSCEPTIBILITY OF GRAM NEGATIVE BACTERIA ISOLATED FROM URINE SAMPLE . Mouau.afribary.org: Retrieved Nov 24, 2024, from https://repository.mouau.edu.ng/work/view/antimicrobial-susceptibility-of-gram-negative-bacteria-isolated-from-urine-sample
MOUAU/11/18883, IHUOMA. "ANTIMICROBIAL SUSCEPTIBILITY OF GRAM NEGATIVE BACTERIA ISOLATED FROM URINE SAMPLE " Mouau.afribary.org. Mouau.afribary.org, 29 Apr. 2020, https://repository.mouau.edu.ng/work/view/antimicrobial-susceptibility-of-gram-negative-bacteria-isolated-from-urine-sample. Accessed 24 Nov. 2024.
MOUAU/11/18883, IHUOMA. "ANTIMICROBIAL SUSCEPTIBILITY OF GRAM NEGATIVE BACTERIA ISOLATED FROM URINE SAMPLE ". Mouau.afribary.org, Mouau.afribary.org, 29 Apr. 2020. Web. 24 Nov. 2024. < https://repository.mouau.edu.ng/work/view/antimicrobial-susceptibility-of-gram-negative-bacteria-isolated-from-urine-sample >.
MOUAU/11/18883, IHUOMA. "ANTIMICROBIAL SUSCEPTIBILITY OF GRAM NEGATIVE BACTERIA ISOLATED FROM URINE SAMPLE " Mouau.afribary.org (2020). Accessed 24 Nov. 2024. https://repository.mouau.edu.ng/work/view/antimicrobial-susceptibility-of-gram-negative-bacteria-isolated-from-urine-sample